9TQ6

Antibody-antigen complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	71.555, 91.277, 142.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.640 - 2.400
R-factor	0.2373
Rwork	0.235
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.044
Data reduction software	XDS (20161101)
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.026	0.404
Number of reflections	37261	3853
<I/σ(I)>	18.9
Completeness [%]	99.8
Redundancy	7.3
CC(1/2)	0.999	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	50 mM imidazole, pH 7, 18% PEG 3350