9TPY
HomA ectodomain (EDC-HomA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.900, 126.600, 155.100 |
| Unit cell angles | 84.50, 84.40, 83.50 |
Refinement procedure
| Resolution | 93.310 - 2.850 |
| R-factor | 0.2411 |
| Rwork | 0.241 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | TNT |
| Refinement software | BUSTER (2.10.4 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.310 | 3.050 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 104620 | 949 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22% PEG 3350, 0.2M Ammonium iodide |
