9TPM
Structure of LolB from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-24 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 94.679, 94.679, 104.942 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.340 - 2.100 |
| R-factor | 0.2295 |
| Rwork | 0.227 |
| R-free | 0.27780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.470 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.248 | 3.818 |
| Rmeas | 0.261 | 4.015 |
| Rpim | 0.080 | 0.922 |
| Number of reflections | 16808 | 1592 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 19.3 | 19.3 |
| CC(1/2) | 0.998 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50 mM Zinc acetate, 50 mM MES pH 6.5, 11% PEG 8000 |
