9TP7

Crystal structure of the C-terminal Ser/Thr phosphatase domain of the Kelch phosphatase BSU1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000029
Spacegroup name	C 1 2 1
Unit cell lengths	226.410, 97.785, 157.799
Unit cell angles	90.00, 129.41, 90.00

Refinement procedure

Resolution	45.380 - 2.100
R-factor	0.1978
Rwork	0.196
R-free	0.22650
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.380	2.220
High resolution limit [Å]	2.100	2.100
Rmeas	0.147	1.873
Number of reflections	155227	24864
<I/σ(I)>	11.44	1.2
Completeness [%]	99.5	99.2
Redundancy	6.8	6.8
CC(1/2)	0.998	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.7 M sodium citrate tribasic dihydrate, 0.1 M Tris [pH 8.0]