9TP5

Crystal structure of the N-terminal Kelch domain of the Kelch phosphatase BSU1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-02
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999874
Spacegroup name	P 43 21 2
Unit cell lengths	77.313, 77.313, 134.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.400 - 2.250
R-factor	0.2227
Rwork	0.222
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.400	2.390
High resolution limit [Å]	2.250	2.250
Rmeas	0.225	3.000
Number of reflections	36873	5942
<I/σ(I)>	12.72	1
Completeness [%]	100.0	100
Redundancy	13.9
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	18 % [w/v] PEG 8,000, 5% [v/v] PEG 550 MME, 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate [pH 6.5]