9TMK
Structure of CYLD CAP-Gly2 bound to Listeria effector protein InlC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.833, 70.010, 97.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.860 - 1.800 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.400 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.083 | 1.310 |
| Rmeas | 0.090 | |
| Number of reflections | 38204 | 2246 |
| <I/σ(I)> | 15.5 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | |
| CC(1/2) | 1.000 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 10% (w/v) PEG 8,000, 100 mM Tris pH 7.0 and 200 mM MgCl2 |
