9TJY

Crystal Structure of the Human Oncogenic Splice Variant Rac1b Fused with the Scaffold Protein POSH (residues 319-348)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.96546
Spacegroup name	P 21 21 2
Unit cell lengths	54.341, 109.618, 46.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.730 - 1.774
Rwork	0.156
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.472
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	1.970
High resolution limit [Å]	1.770	1.770
Rmerge	0.115	1.740
Rmeas	0.120	1.820
Rpim	0.033	0.520
Number of reflections	18728	936
<I/σ(I)>	12.1	1.7
Completeness [%]	93.0	64
Redundancy	12.9	11.5
CC(1/2)	0.997	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	14% 1-propanol, 10% PEG 5000 MME, 0.1 M MES pH 6.8