9TG7

Crystal structure of beta-TrCP bound by monophosphorylated WEE1 degron peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	45.894, 47.307, 148.272
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.140 - 1.684
R-factor	0.2099
Rwork	0.208
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.136	1.991
High resolution limit [Å]	1.684	1.684
Number of reflections	21819	1091
<I/σ(I)>	5.1
Completeness [%]	90.1
Redundancy	6.9
CC(1/2)	0.979	0.157

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	20% PEG10K, 0.1 M Na-Hepes pH 7.5