9TFY

Arabidopsis thaliana Casein Kinase 2 (CK2) alpha1 - beta1 complex bound to inositol hexakisphosphate (InsP6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000040
Spacegroup name	I 41 2 2
Unit cell lengths	210.189, 210.189, 272.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.070 - 5.500
R-factor	0.2487
Rwork	0.248
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.070	5.830
High resolution limit [Å]	5.500	5.500
Rmeas	0.267	6.030
Number of reflections	10252	1622
<I/σ(I)>	13.01	1.3
Completeness [%]	99.8	100
Redundancy	26.6	27.9
CC(1/2)	0.998	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	0.1 M Carboxylic acids (0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M sodium citrate tribasic dihy drate, 0.2 potassium sodium tartrate tetrahydrate, 0.2 M sodium oxamate), 0.1 M buffer system 2 (HEPES, MOPS, pH 7.2), 31% PrecipMix 2 (40% [v/v] Ethylene glycol, 20% [w/v] PEG 4,000, 20 % [w/v] PEG 3,350, 0.2 M sodium malonate, 0.1 M Bis-Tris [pH 6.5] (Morpheus G2, Molecular Dimensions)