9TFX
Arabidopsis thaliana Casein Kinase 2 (CK2) alpha isoform 1 in complex with inositol hexakisphosphate (InsP6)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000036 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 61.903, 63.897, 97.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.690 - 1.700 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.713 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.690 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.133 | 2.320 |
| Number of reflections | 43248 | 6885 |
| <I/σ(I)> | 17.1 | 1.25 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 12.8 | 13 |
| CC(1/2) | 1.000 | 0.442 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 10% (w/v) PEG 6,000, 0.1 M HEPES pH 6.5 |
