9TFW

Arabidopsis thaliana Casein Kinase 2 (CK2) alpha isoform 3 apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9999951402559115
Spacegroup name	P 21 21 21
Unit cell lengths	53.979, 59.556, 107.013
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.190 - 1.250
R-factor	0.1694
Rwork	0.169
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.005
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.190	1.320
High resolution limit [Å]	1.250	1.250
Rmeas	0.087	2.712
Number of reflections	96088	15218
<I/σ(I)>	16.5	1.02
Completeness [%]	99.6	98.4
Redundancy	12.9	12.3
CC(1/2)	1.000	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	20 % [w/v] PEG 3,350, 0.2 M sodium malonate, 0.1 M Bis-Tris [pH 6.5]