9TFT
Complex of blast (Magnaporthe oryzae) effector PWT7 with the engineered integrated HMA domain of Rwt7 from wheat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.750006 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.524, 66.174, 65.747 |
| Unit cell angles | 90.00, 104.85, 90.00 |
Refinement procedure
| Resolution | 63.630 - 0.990 |
| R-factor | 0.14087 |
| Rwork | 0.140 |
| R-free | 0.15265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.948 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.170 | 1.010 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.063 | 1.660 |
| Rmeas | 0.066 | 1.723 |
| Rpim | 0.017 | 0.461 |
| Total number of observations | 2555845 | 125512 |
| Number of reflections | 186021 | 9174 |
| <I/σ(I)> | 15.2 | 1.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.7 | 13.7 |
| CC(1/2) | 1.000 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl pH 8.5, 25% (w/v) PEG 6000 |
