9TFQ
Complex of blast (Magnaporthe oryzae) effectors PWT7 and PBY2 with the engineered integrated HMA domain of Rmo2 from barley
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-06 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.953727 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.592, 30.590, 61.448 |
| Unit cell angles | 90.00, 93.02, 90.00 |
Refinement procedure
| Resolution | 61.360 - 1.400 |
| R-factor | 0.15306 |
| Rwork | 0.151 |
| R-free | 0.18896 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.724 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.360 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.056 | 1.330 |
| Rmeas | 0.058 | 1.380 |
| Rpim | 0.016 | 0.367 |
| Total number of observations | 566095 | 27819 |
| Number of reflections | 41916 | 2032 |
| <I/σ(I)> | 19.8 | 1.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.5 | 13.7 |
| CC(1/2) | 1.000 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M KBr, 30% (w/v) PEG2000 MME |
