9TFP
Complex of blast (Magnaporthe oryzae) effector PWT7 with the integrated HMA domain of Rwt7 from wheat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-26 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.953738 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.915, 73.609, 127.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.850 - 1.600 |
| R-factor | 0.16298 |
| Rwork | 0.161 |
| R-free | 0.19766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.776 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.850 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 1.043 |
| Rmeas | 0.054 | 1.063 |
| Rpim | 0.011 | 0.201 |
| Total number of observations | 67667 | |
| Number of reflections | 50590 | 2424 |
| <I/σ(I)> | 32.7 | 2 |
| Completeness [%] | 100.0 | |
| Redundancy | 27 | 27.9 |
| CC(1/2) | 1.000 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.2, 16% (w/v) PEG 3350 |
