9TFO
Complex of blast (Magnaporthe oryzae) effector PBY2 with the integrated HMA domain of Rmo2 from barley
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-26 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.953731 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 89.793, 89.793, 89.088 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.880 - 2.200 |
| R-factor | 0.21333 |
| Rwork | 0.210 |
| R-free | 0.26962 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.679 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.090 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.931 |
| Rmeas | 0.063 | 0.977 |
| Rpim | 0.019 | 0.295 |
| Number of reflections | 21089 | 1838 |
| <I/σ(I)> | 26.6 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.8 | 21.1 |
| CC(1/2) | 1.000 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.6 M ammonium sulfate, 0.1 M sodium acetate pH 4.2 |
