9TEU

Oligomerisation of type III CRISPR-associated Csx15 to regulate antiviral signalling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.7918
Spacegroup name	C 1 2 1
Unit cell lengths	83.330, 32.530, 57.750
Unit cell angles	90.00, 132.83, 90.00

Refinement procedure

Resolution	30.560 - 0.900
R-factor	0.157
Rwork	0.156
R-free	0.16800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.188
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	0.920
High resolution limit [Å]	0.900	0.900
Rmerge	0.036
Number of reflections	79628	2317
<I/σ(I)>	16.8
Completeness [%]	94.6
Redundancy	6.2
CC(1/2)	1.000	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris pH 8.5 22 % v/v PEG Smear Broad