9TET

Oligomerisation of type III CRISPR-associated Csx15 to regulate antiviral signalling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 62
Unit cell lengths	89.310, 89.310, 31.413
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.660 - 1.880
R-factor	0.1996
Rwork	0.198
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.848
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.345	1.910
High resolution limit [Å]	1.880	1.880
Number of reflections	11875	582
<I/σ(I)>	18.2
Completeness [%]	99.8
Redundancy	40
CC(1/2)	1.000	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium/potassium phosphate pH 7.5 0.1 M HEPES 7.5 22.5 % v/v PEG smear Medium 10 % v/v Glycerol