9TEL
Structure of chicken LGP2 bound to 10-mer RNA mismatched duplex that mimics the influenza B virus vRNA promoter (panhandle) and to ADP-AlF4-Mg.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.900, 96.462, 122.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.744 - 1.436 |
| Rwork | 0.148 |
| R-free | 0.17850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.242 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.744 | 1.557 |
| High resolution limit [Å] | 1.436 | 1.436 |
| Rmerge | 0.052 | 0.719 |
| Number of reflections | 117940 | 5897 |
| <I/σ(I)> | 11.2 | 1.6 |
| Completeness [%] | 78.1 | 18.2 |
| Redundancy | 3.8 | 4 |
| CC(1/2) | 0.997 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Directly after size exclusion chromatography, chLGP2 was mixed with duplex RNA in a 1:1 ratio and incubated for 30 minutes on ice. The complexes were concentrated to around 10 mg/ml and then 2 mM ADP:AlF4 and 2 mM MgCl2 were added. The complex was mixed with reservoir solution (0.1M Na citrate pH 6.1 0.46 M NH4 dihydrophosphate) in a ratio 1:2. |
