9TEK

Lysozyme crystal structure by beam sweeping serial crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	EUROPEAN XFEL BEAMLINE SPB/SFX
Synchrotron site	European XFEL
Beamline	SPB/SFX
Temperature [K]	298
Detector technology	PIXEL
Collection date	2024-09-23
Detector	PSI JUNGFRAU 4M
Wavelength(s)	1.36245
Spacegroup name	P 43 21 2
Unit cell lengths	79.030, 79.030, 37.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.340 - 2.000
R-factor	0.2122
Rwork	0.207
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.927
Data reduction software	CrystFEL (0.11.1)
Data scaling software	CrystFEL (0.11.1)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.340	2.130
High resolution limit [Å]	2.000	2.000
Number of reflections	8478	1367
<I/σ(I)>	12.39	6.31
Completeness [%]	100.0	100
Redundancy	1075.9	239.6
CC(1/2)	0.970	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	1M NaCl, 40% (v/v) ethylene glycol, 15% (w/v) PEG4000, 50 mM sodium acetate pH 3.5