9TEJ

Lysozyme crystal structure by beam sweeping serial crystallography with pink beam

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	298
Detector technology	PIXEL
Collection date	2024-03-08
Detector	PSI JUNGFRAU 1M
Wavelength(s)	0.8123-0.8453
Spacegroup name	P 43 21 2
Unit cell lengths	78.990, 78.990, 37.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.330 - 2.100
R-factor	0.225
Rwork	0.221
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.955
Data reduction software	CrystFEL (0.11.1)
Data scaling software	CrystFEL (0.11.1)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.330	2.260
High resolution limit [Å]	2.100	2.100
Number of reflections	7321	1420
<I/σ(I)>	6.8	4.04
Completeness [%]	99.8	99.93
Redundancy	316.4	308.4
CC(1/2)	0.950	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1M NaCl, 35% (v/v) ethylene glycol, 20% (w/v) PEG4000, 50 mM sodium acetate pH 3.5