9TDZ

Crystal structure of beta-TrCP bound by diphosphorylated claspin degron peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 21 21 21
Unit cell lengths	44.498, 46.859, 150.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.280 - 1.346
R-factor	0.179
Rwork	0.177
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.170
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.071	1.495
High resolution limit [Å]	1.346	1.346
Number of reflections	49954	2498
<I/σ(I)>	6.7
Completeness [%]	92.2
Redundancy	6.8
CC(1/2)	0.996	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG4K, 0.2 M ammonium sulfate, 0.1 M sodium citrate pH 5.6