9TDK

Structure of an LPMO expressed in E.coli (LsAA9A) at 2.72x10^6 Gy

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8731
Spacegroup name	P 41
Unit cell lengths	49.210, 49.210, 109.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.953 - 1.650
Rwork	0.168
R-free	0.19080
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.553
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.953	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	31311	3056
<I/σ(I)>	3.7
Completeness [%]	99.8	99.7
Redundancy	14	13.7
CC(1/2)	0.994	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	278	20 mM sodium acetate pH 5.5, 150 mM sodium chloride