9TDJ
Structure of an LPMO expressed in E.coli (LsAA9A) at 2.73x10^6 Gy
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.081, 48.081, 109.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.022 - 1.861 |
| Rwork | 0.210 |
| R-free | 0.25810 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.532 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.022 | 1.900 |
| High resolution limit [Å] | 1.861 | 1.861 |
| Number of reflections | 20742 | 1273 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 13.4 | 13.5 |
| CC(1/2) | 0.987 | 0.471 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 20 mM sodium acetate pH 5.5, 150 mM sodium chloride |
