9TDI
Structure of an LPMO expressed in E.coli (LsAA9A) at 2.80x10^6 Gy
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.8551 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.283, 48.283, 109.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.283 - 1.060 |
| Rwork | 0.143 |
| R-free | 0.15260 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.945 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.283 | 1.100 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Number of reflections | 112352 | 10599 |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 99.5 | 95.3 |
| Redundancy | 12.1 | 5.8 |
| CC(1/2) | 1.000 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 20 mM sodium acetate pH 5.5, 150 mM sodium chloride |
