9TDF
Structure of an LPMO expressed in E.coli (LsAA9A) merged from multiple crystals with an average dose of 88.5kGy at room temperature
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE MicroMAX |
| Synchrotron site | MAX IV |
| Beamline | MicroMAX |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2024-10-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9544 |
| Spacegroup name | P 41 |
| Unit cell lengths | 49.660, 49.660, 109.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.660 - 1.850 |
| Rwork | 0.179 |
| R-free | 0.21220 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.660 | 1.980 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 21161 | 3818 |
| <I/σ(I)> | 3.35 | |
| Completeness [%] | 93.6 | 91.9 |
| Redundancy | 2.8 | 2.87 |
| CC(1/2) | 0.963 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 25 mM sodium acetate pH 5.5, 20 mM sodium chloride |
