9TD6

Structure of an LPMO expressed in E.coli (LsAA9A) at 4.60x10^4 Gy

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8551
Spacegroup name	P 41
Unit cell lengths	48.691, 48.691, 109.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.691 - 2.220
Rwork	0.215
R-free	0.23660
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.514
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.691	2.300
High resolution limit [Å]	2.220	2.220
Number of reflections	12572	1220
<I/σ(I)>	2.4
Completeness [%]	100.0	99.9
Redundancy	11.5
CC(1/2)	0.985	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	278	20 mM sodium acetate pH 5.5, 150 mM sodium chloride