9TD0

Structure of an LPMO expressed in E.coli (LsAA9A) at 5.27x10^4 Gy

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 41
Unit cell lengths	48.290, 48.290, 109.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.337 - 2.070
Rwork	0.175
R-free	0.22140
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.736
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.340	2.120
High resolution limit [Å]	2.070	2.070
Number of reflections	15289	1120
<I/σ(I)>	6.73
Completeness [%]	100.0	100
Redundancy	10.8
CC(1/2)	0.994	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	278	20 mM sodium acetate pH 5.5, 150 mM sodium chloride