9TCZ

Structure of an LPMO expressed in E.coli (LsAA9A) at 6.40x10^4 Gy

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 41
Unit cell lengths	48.070, 48.070, 108.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.116 - 2.060
Rwork	0.187
R-free	0.23960
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.587
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.120	2.110
High resolution limit [Å]	2.060	2.060
Number of reflections	15277	1116
<I/σ(I)>	6.63
Completeness [%]	100.0	99.8
Redundancy	12.8
CC(1/2)	0.995	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	278	20 mM sodium acetate pH 5.5, 150 mM sodium chloride