9TCV

Structure of an LPMO expressed in E.coli (LsAA9A) at 5.37x10^4 Gy

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8731
Spacegroup name	P 41
Unit cell lengths	49.472, 49.472, 109.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.472 - 1.850
Rwork	0.194
R-free	0.22770
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.509
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.620	1.890
High resolution limit [Å]	1.850	1.850
Number of reflections	22367	1271
<I/σ(I)>	8.1
Completeness [%]	99.5
Redundancy	13.6
CC(1/2)	0.995	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	278	20 mM sodium acetate pH 5.5, 150 mM sodium chloride