9TBU

E. coli EF-Tu-T62A:GDP AMPylated at T65

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-27
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.68879
Spacegroup name	P 1 21 1
Unit cell lengths	42.746, 76.700, 60.380
Unit cell angles	90.00, 91.84, 90.00

Refinement procedure

Resolution	60.350 - 1.230
R-factor	0.1763
Rwork	0.175
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.963
Data reduction software	XDS (VERSION Jun 30, 2023 BUILT=20230630)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.350	1.354
High resolution limit [Å]	1.228	1.228
Rmerge	0.072	0.974
Number of reflections	69905	3496
<I/σ(I)>	13.7	1.6
Completeness [%]	89.0
Redundancy	6.9
CC(1/2)	0.999	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.15 M Ammonium Sulfate, 21 % (w/v) PEG 3350