9T91

XN-IL lectin from Xenorhabdus nematophila in complex with hyaluronan tetrasaccharide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.6888
Spacegroup name	P 31 2 1
Unit cell lengths	65.797, 65.797, 129.985
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.883 - 1.600
Rwork	0.182
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.820
Data reduction software	autoPROC
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.330	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.079	1.373
Rmeas	0.081	1.407
Rpim	0.018	0.304
Number of reflections	43532	6216
<I/σ(I)>	19.4	2.4
Completeness [%]	99.6	99.2
Redundancy	20.5	20.9
CC(1/2)	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	MES, ethylene glycol