9T8G

Shewanella oneidensis EF-Tu:GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-09
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	63.485, 76.737, 98.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.470 - 1.610
R-factor	0.1798
Rwork	0.178
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.158
Data reduction software	XDS (VERSION Jun 30, 2023 BUILT=20230630)
Data scaling software	STARANISO (2.3.74)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.470	1.730
High resolution limit [Å]	1.610	1.610
Rmerge	0.077	1.621
Number of reflections	45515	2276
<I/σ(I)>	16.9	1.4
Completeness [%]	94.7	61.4
Redundancy	13.3
CC(1/2)	1.000	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.18 M Sodium Acetate, 21,7 % PEG 3350