9T6R

Crystal Structure of the Listeria monocytogenes CadC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-22
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.8856
Spacegroup name	P 32 2 1
Unit cell lengths	109.768, 109.768, 124.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.550 - 3.010
R-factor	0.2627
Rwork	0.260
R-free	0.31550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.328
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.21.2_5419)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.550	3.170
High resolution limit [Å]	3.010	3.010
Rmerge	0.252	1.193
Rmeas	0.282	1.441
Rpim	0.127	0.790
Number of reflections	17557	4535
<I/σ(I)>	5.12	0.96
Completeness [%]	99.7	98.86
Redundancy	4.8	3
CC(1/2)	0.994	0.138

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	8% Tacsimate pH 5.0, 20% PEG 3350, 30% Glycerol