9T4Q
Structure of the SUN4 domain of SIM1 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.037, 78.639, 80.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.920 - 1.230 |
| R-factor | 0.1454 |
| Rwork | 0.144 |
| R-free | 0.16590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.013 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.920 | 1.270 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.062 | 1.550 |
| Number of reflections | 74828 | 7197 |
| <I/σ(I)> | 14.5 | 1.5 |
| Completeness [%] | 98.3 | 95.9 |
| Redundancy | 6.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 281 | 20 mg/ml, 0.1 M MgCl2, 0.1 M Tris-HCl, 30 % PEG 4000 |
