9T4O
Structure of the E283A mutant of the SUN4 domain from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.330, 99.090, 101.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 1.240 |
| R-factor | 0.1321 |
| Rwork | 0.131 |
| R-free | 0.15620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.370 | 1.300 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.049 | 0.640 |
| Number of reflections | 88813 | 8619 |
| <I/σ(I)> | 13.8 | 2.7 |
| Completeness [%] | 99.8 | 97.9 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 281 | 50 mg/ml, 40% PEG400, 0.2 M MgCl2, 0.1 M MES |
