9T47

Structure of the SUN4 domain from Saccharomyces cerevisiae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0332
Spacegroup name	I 2 2 2
Unit cell lengths	62.400, 99.160, 102.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 1.100
R-factor	0.1243
Rwork	0.123
R-free	0.14160
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.720	1.110
High resolution limit [Å]	1.050	1.050
Rmerge	0.049	0.535
Number of reflections	143630	11649
<I/σ(I)>	19.9	2.5
Completeness [%]	97.7	85.8
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	281	50 mg/ml ScSUN4, 10% PEG 400, 0.1 M NaHEPES pH 7.5, 1.5 M ammonium sulphate