9T46
Crystal structure of the Fab 40G5c in complex with a CD3 epsilon peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.519, 74.045, 104.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.420 - 1.650 |
| R-factor | 0.1852 |
| Rwork | 0.183 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS (v20180409) |
| Data scaling software | Aimless (v0.7.3) |
| Phasing software | PHASER (v.2.8.2) |
| Refinement software | PHENIX (v1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.519 | 1.763 |
| High resolution limit [Å] | 1.650 | 1.757 |
| Rmerge | 0.135 | 2.602 |
| Rmeas | 0.147 | 2.825 |
| Rpim | 0.058 | 1.091 |
| Total number of observations | 3509 | |
| Number of reflections | 49841 | 525 |
| <I/σ(I)> | 7.7 | 0.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.1 M sodium acetate, pH 4.6, 2 M (NH4)2SO4 |
