9T3L

Crystal structure of the Acl1 ankyrin repeat domain in complex with the second Rpl1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967697
Spacegroup name	P 65 2 2
Unit cell lengths	101.650, 101.650, 108.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.070 - 2.550
R-factor	0.197
Rwork	0.195
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.070	2.700
High resolution limit [Å]	2.550	2.550
Rmeas	0.295	2.220
Number of reflections	11261	1761
<I/σ(I)>	17.87
Completeness [%]	100.0
Redundancy	39.52
CC(1/2)	0.998	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 mM ammonium acetate, 0.1 mM sodium citrate (pH 5.6), 30% (w/v) PEG 4000