9T3F

RACB with GSP and the fragment of RIPb protein bound (parallel)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.03320
Spacegroup name	P 61 2 2
Unit cell lengths	64.000, 64.000, 243.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.800 - 2.070
R-factor	0.20752
Rwork	0.205
R-free	0.24691
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.672
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.070	2.070
Number of reflections	18023	1279
<I/σ(I)>	24.5	1.1
Completeness [%]	99.9	99.9
Redundancy	19.1
CC(1/2)	0.999	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	20 % w/v PEG 6,000, 100 mM HEPES pH 7.0, 200 mM Sodium Chloride