9T3E
RACB with GSP and the fragment of RIPb protein bound (antiparallel)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-12 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.510, 139.450, 60.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.530 - 2.300 |
| R-factor | 0.2618 |
| Rwork | 0.259 |
| R-free | 0.31750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.092 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 14643 | 1063 |
| <I/σ(I)> | 19.5 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.4 | 13.2 |
| CC(1/2) | 1.000 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 10 mM Magnesium Chloride, 50 mM MES pH 5.6, 1.8 M Lithium Sulfate |
