9T3D
RACB with GPPNHP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-06 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.710, 66.700, 37.100 |
| Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
| Resolution | 42.810 - 1.330 |
| R-factor | 0.11768 |
| Rwork | 0.116 |
| R-free | 0.13948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.848 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.360 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 47328 | 3247 |
| <I/σ(I)> | 21.5 | 3.2 |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 6.8 | |
| CC(1/2) | 1.000 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 300 mM Sodium Fluoride and 18% (w/v) PEG 3350 |
