9T3C
RACB with GDT bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-06 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.320, 98.320, 107.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.320 - 2.030 |
| R-factor | 0.17329 |
| Rwork | 0.173 |
| R-free | 0.19660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.755 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 39262 | 2789 |
| <I/σ(I)> | 9.7 | 1.2 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 8.1 | |
| CC(1/2) | 0.991 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 0.18 M Magnesium Formate, 0.1 M Sodium Acetate pH 4.0 and 17% (w/v) PEG 5,000 MME |
