9T2Z
Spindlin 1 with crystallization epitope mutations H127D:L128D:T131R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.880, 99.910, 45.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.950 - 1.870 |
| R-factor | 0.2599 |
| Rwork | 0.259 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.280 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.175 | 0.808 |
| Number of reflections | 24861 | 1814 |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.2M ammonium sulfate 25% PEG3350 0.1M HEPES pH 7.5 |
