9T2W
YdhG-like domain-containing protein (Frataxin) from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-28 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.974, 51.296, 60.583 |
| Unit cell angles | 90.00, 107.17, 90.00 |
Refinement procedure
| Resolution | 57.880 - 1.750 |
| Rwork | 0.180 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.469 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.880 | 1.752 |
| High resolution limit [Å] | 1.720 | 1.722 |
| Rmerge | 0.173 | 3.009 |
| Rmeas | 0.188 | 3.297 |
| Rpim | 0.072 | 1.330 |
| Number of reflections | 27261 | 1336 |
| <I/σ(I)> | 6.9 | 1.4 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 6.8 | 6 |
| CC(1/2) | 0.966 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 295.15 | 2.5 mg/mL protein, 0.1 M MES. 10% PEG4000 |
