9T0N

Crystal structure of TagF (ACIAD2683) from Acinetobacter baylyi ADP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000009
Spacegroup name	P 21 21 21
Unit cell lengths	45.146, 74.909, 85.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.340 - 1.800
R-factor	0.2113
Rwork	0.209
R-free	0.24520
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.705
Data reduction software	DIALS (2.2)
Data scaling software	Aimless (0.5.21)
Phasing software	CRANK2
Refinement software	PHENIX ((2.0_5716))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.500	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	51891	1591
<I/σ(I)>	7.4	0.5
Completeness [%]	100.0	100
Redundancy	12.1	12.3
CC(1/2)	0.998	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.162 M CdSO4, 0.1 M Hepes 7.5 pH, 0.5 M NaOAc pH 8