9SZM
Human fumarylacetoacetate hydrolase (FAH) in complex with 3C10
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.668, 105.369, 75.020 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 74.480 - 1.800 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.845 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.480 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 93562 | 13473 |
| <I/σ(I)> | 15.4 | 8.9 |
| Completeness [%] | 98.3 | 97.3 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.994 | 0.977 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Bis-Tris pH 5.5 and 7.0 at 25% to PEG 3350. |
