9SXC

Crystal structure of the Legionella pneumophila effector SidL (Lpg0437) in complex with its metaeffector LegA11 (Lpg0436)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 21
Unit cell lengths	68.530, 93.380, 133.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.850 - 2.400
Rwork	0.224
R-free	0.26750
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.850	3.400	2.486
High resolution limit [Å]	2.400	3.300	2.400
Rmeas	0.142	2.020	1.411
Number of reflections	41606	2217	5384
<I/σ(I)>	23.42
Completeness [%]	99.7	100	100
Redundancy	6.9	28.3	7
CC(1/2)	0.999	0.892	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Protein:12 mg/mL SidL, 5.2 mg/mL LegA11, 0.34 mg/mL Trypsin Reservoir: 100 mM HEPES-NaOH pH 7.5, 200 mM L-proline 140 g/L PEG3350
1	VAPOR DIFFUSION, SITTING DROP		291	Protein:12 mg/mL SidL, 5.2 mg/mL LegA11, 0.34 mg/mL Trypsin Reservoir: 100 mM HEPES-NaOH pH 7.5, 200 mM L-proline 140 g/L PEG3350