9SX3

Middle domain of HSP90C from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	270
Detector technology	PIXEL
Collection date	2022-01-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	C 1 2 1
Unit cell lengths	132.010, 84.030, 60.370
Unit cell angles	90.00, 95.88, 90.00

Refinement procedure

Resolution	47.090 - 3.010
R-factor	0.2057
Rwork	0.203
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.183
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.719	2.965
High resolution limit [Å]	2.915	2.915
Number of reflections	14455	727
<I/σ(I)>	13.2
Completeness [%]	98.8
Redundancy	6.91
CC(1/2)	0.998	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		282	0.2 M lithium sulfate, 0.1 M Tris pH 8.5, 40% polyethylene glycol (PEG) 400