9SWT

Middle and C-terminal domains of HSP90C from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	270
Detector technology	PIXEL
Collection date	2022-01-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	C 2 2 21
Unit cell lengths	82.280, 170.620, 135.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.060 - 3.150
R-factor	0.2201
Rwork	0.217
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.688
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_4933: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.112	3.561
High resolution limit [Å]	3.147	3.147
Number of reflections	235265	486
<I/σ(I)>	14.2
Completeness [%]	91.1
Redundancy	24.2
CC(1/2)	1.000	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		282	20 mM magnesium chloride, 0.1 M HEPES pH 7.5, 28% polyacrylic acid, ethylene glycol 9%