9SRG
Prenylated FMN oxidative maturase PhdC, FMN-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-08-10 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 59.732, 75.795, 79.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.830 - 1.320 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | AutoProcess |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.830 | 1.520 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Number of reflections | 27304 | 1365 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 89.3 | 39.3 |
| Redundancy | 13 | 7.7 |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 12 % w/v PEG 6000, 0.1 M Sodium citrate, pH 5.6, 0.1 M Lithium sulfate |
